Estimation of clearance of 99mTc-DTPA with the methods of gamma- and fluid-scintillation spectrometry for calculation of rate of glomerular filtration

Estimation of glomelular filtration rate (GFR) has the special importance because it can provide the information on damage of glomerulus and can be the one of the major early criterions characterized the normal function of kidneys. Width-available methods of GFR estimation can not always give valid results. Methods with the usage of radiopharm drugs (RPD) allows to perform the estimation of GFR with the high accuracy that has the big clinical significance.

Aim of the work – to test the protocols of GFR estimation with the help of the clearance of single infused RPD 99mTc-DTPA and reveal the correlation between GFR estimated by the clearance of 99mTc-DTPA calculated with gamma spectrometric method (GS) and liquid scintillation spectrometry (LSS).

Twenty patients were enrolled. Blood samples collection was made after 1, 2 and 3 hours after intravenous infusion of 99mTc-DTPA. Estimation was made by the LSS method and with the help of GS. GFR was calculated as rate of infused dose D0 to surface under the curve of excretion S.

Our results suggest that the previous published data is correct. This data shows that for correct calculation of GFR is enough to do the estimation of drug clearance in blood samples received after 1, 2 and 3 hours after infusion of RPD.

Thus, the protocols of GFR estimation by the clearance of single infused RPD by the methods of GS and LSS were tested. GFR data calculated by 99mTc-DTPA clearance with the help of method LSS did not showed any differences in comparison with GS method.

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