Research of the intracranial neoplasia cell sensitivity to chemotherapy drugs
https://doi.org/10.17650/2311-1267-2015-2-4-51-57
Abstract
Introduction. The article is devoted to the personalized selection of chemotherapy for intracranial tumor cell cultures obtained from patients.
Materials and methods. In the primary cell cultures of intracranial tumors (pilocytic astrocytoma, anaplastic astrocytoma, glioblastoma and medulloblastoma), taken from 98 patients aged 3 months to 17 years, were evaluated their sensitivity to chemotherapeutic drugs (vincristine, carboplatin, methotrexate, temozolomide, cytarabine, cyclophosphamide, cisplatin and etoposide) by staining the cells with trypan blue in Goryaev chamber. Using principal component analysis and two-factor analysis of variance was adjusted depending on the chemosensitivity factor ‟patient”, the type of tumor, and the mechanism of action of chemotherapy.
Results. Based on cell sensitivity to chemotherapeutic drugs neuroepithelial tumors particular patient applying statistical methods of analysis allows to identify individual patients at high, moderate or low chemosensitivity, patients with chemotherapy and effectively act unidirectionally or differently and have weak cytotoxic effect on the tumor. Application of the method of two-way analysis of variance revealed that the main factor affecting the sensitivity of tumor cells to chemotherapeutic drugs is a factor of the ‟patient”.
Conclusions. Application of principal component creates a scientific basis for the selection of individual schemes (protocols) therapy in this group of patients.
About the Authors
A. N. Chernov
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
28 Akademicheskaya St., Minsk, 220072,
Belarus
28 Akademicheskaya St., Minsk, 220072,
N. N. Yatskov
Belarusian State University
Belarus
Belarus
4 Prosp. Nezavisimosti, Minsk, 220030
V. V. Skakun
Belarusian State University
Belarus
4 Prosp. Nezavisimosti, Minsk, 220030
Belarus
4 Prosp. Nezavisimosti, Minsk, 220030
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Review
For citations:
Chernov A.N., Yatskov N.N., Skakun V.V. Research of the intracranial neoplasia cell sensitivity to chemotherapy drugs. Russian Journal of Pediatric Hematology and Oncology. 2015;2(4):51-57. (In Russ.) https://doi.org/10.17650/2311-1267-2015-2-4-51-57
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